APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml for 30 minutes at RT |
2-4μg/ml (for coating) |
Assay dependent |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
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Not tested in other applications.
Calculated MW
Product Note
This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD71 , IMD46 , T9 , TFR , TFR1 , TFRC , TR , TRFR , p90 , transferrin receptor , Transferrin receptor 1 , mammary tumor virus receptor 1
Cellular Localization
Cell membrane,Melanosome,Secreted
Background
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
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GTX18020 IHC-P Image
IHC-P analysis of human placenta tissue using GTX18020 CD71 antibody [TFRC/1839].
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GTX18020 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD71 Mouse Monoclonal Antibody (TFRC/1839). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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REFERENCE
There are currently no references for CD71 antibody [TFRC/1839] (GTX18020). Be the first to share your publications with this product.
REVIEW
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